Journal: Transplantation

Article Title: Evidence to support a contribution of polyreactive antibodies to HLA serum reactivity

doi: 10.1097/TP.0000000000000840

Figure Lengend Snippet: The reactivity of non-polyreactive antibody 3D4 and polyreactive antibodies 2B4, 2D9, 4G4 and 4G10 to HLA class I antigens was tested by Luminex using single antigen beads. Each bar represents reactivity to one corresponding class I molecule. Antigens are grouped according to their loci (A, B and C).

Article Snippet: Adsorbed IgG were then assessed for their reactivity to HLA molecules by Luminex (Labscreen and SAB).

Techniques: Luminex

Journal: Transplantation

Article Title: Evidence to support a contribution of polyreactive antibodies to HLA serum reactivity

doi: 10.1097/TP.0000000000000840

Figure Lengend Snippet: The reactivity of non-polyreactive antibody 3D4 and polyreactive antibodies 2B4, 2D9, 4G4 and 4G10 to HLA class I antigens was tested by Luminex using regular single antigen beads (orange bars), iBEADS (blue bars) and regular single antigen beads treated with acid (green bars). Each bar represents reactivity to one corresponding class I molecule labeled below. Only the 50 antigens towards which the supernatants reacted the most are depicted for each clone. Antigens are sorted based on the supernatant reactivity to regular single antigen beads. Complete reactivity profiles are reported in supplementary Figure S1-S5.

Article Snippet: Adsorbed IgG were then assessed for their reactivity to HLA molecules by Luminex (Labscreen and SAB).

Techniques: Luminex, Labeling

Journal: Transplantation

Article Title: Evidence to support a contribution of polyreactive antibodies to HLA serum reactivity

doi: 10.1097/TP.0000000000000840

Figure Lengend Snippet: (A) Comparison of IgG reactivity to apoptotic cells in pre-transplant patients. Patients whose serum reacted to HLA Class I, HLA Class II, or MICA (MFI ≥ 1000) were separated from patients whose serum did not react strongly (MFI < 1000). The IgG reactivity to apoptotic cells (Log2 MFI) was compared between the 2 groups. The numbers of samples analyzed in each group are shown below the box plot. P values from two-tailed Mann Whitney tests are shown for corresponding box pairs. The horizontal bar represents the median value; the bottom and top of each box represent the 25th and 75th percentiles; the lower and upper bars of each box represent the minimum and maximum values. (B) Correlation between IgG reactivity to apoptotic cells and IgG reactivity to HLA or MICA in pre-transplant patients (N=300). IgG reactivity to apoptotic Jurkat cells (X axis) and reactivity to HLA class I, HLA class II or MICA (Y axis) are depicted as log2 MFI and log2 (10+MFI), respectively. Each circle represents the serum of one patient. Dots corresponding to sera reactive to HLA class I, class II or MICA (>1000) are colored in green or red if their reactivity to apoptotic cells was below or above the median respectively. P values from Spearman correlation tests are shown for corresponding groups.

Article Snippet: Adsorbed IgG were then assessed for their reactivity to HLA molecules by Luminex (Labscreen and SAB).

Techniques: Two Tailed Test, MANN-WHITNEY

Journal: Transplantation

Article Title: Evidence to support a contribution of polyreactive antibodies to HLA serum reactivity

doi: 10.1097/TP.0000000000000840

Figure Lengend Snippet: Serum reactivity to HLA class I and class II molecules was assessed by Luminex using single antigen beads. HLA class I and class II PRA were calculated by dividing the number of beads recognized by the total number of corresponding class I or class II beads. All redundant beads were counted as one in the PRA calculation. Patients were separated into two groups depending on IgG reactivity to apoptotic cells below or above the median value. P values from two-tailed Mann Whitney tests are shown for corresponding group pairs. The horizontal bar represents the median value.

Article Snippet: Adsorbed IgG were then assessed for their reactivity to HLA molecules by Luminex (Labscreen and SAB).

Techniques: Luminex, Two Tailed Test, MANN-WHITNEY

Journal: Transplantation

Article Title: Evidence to support a contribution of polyreactive antibodies to HLA serum reactivity

doi: 10.1097/TP.0000000000000840

Figure Lengend Snippet: (A) Reactivity of sera from 20 kidney transplant recipients to Class I, Class II, and MICA was measured by Luminex (Labscreen mixed beads) before and after adsorption on apoptotic cells. For clarity, sera for which the reactivity was not reduced by adsorption (<25% decrease; upper panels) are presented separately from sera for which the reactivity was significantly decreased after adsorption (≥ 25%, lower panel). (B) The reactivity of three representative sera to HLA Class I was measured by Luminex (SAB) before and after adsorption on apoptotic cells. Only the 25 antigens towards which the supernatants reacted the most are depicted. Antigens are sorted based on the supernatant reactivity to regular single antigen beads.

Article Snippet: Adsorbed IgG were then assessed for their reactivity to HLA molecules by Luminex (Labscreen and SAB).

Techniques: Luminex, Adsorption